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Updated: Jul 22, 2026

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
Published on: January 26, 2012
Detection of elementary flux modes in biochemical networks: a promising tool for pathway analysis and metabolic
S Schuster1, T Dandekar, D A Fell
1Department of Bioinformatics, Max Delbrück Centre for Molecular Medicine, Berlin-Buch, Germany. schuster@bp.biologie.hu-berlin.de
Abstract:
Rational metabolic engineering requires powerful theoretical methods such as pathway analysis, in which the topology of metabolic networks is considered. All metabolic capabilities in steady states are composed of elementary flux modes, which are minimal sets of enzymes that can each generate valid steady states. The modes of the fructose-2,6-bisphosphate cycle, the combined tricarboxylic-acid-glyoxylate-shunt system and tryptophan synthesis are used here for illustration. This approach can be used for many biotechnological applications such as increasing the yield of a product, channelling a product into desired pathways and in functional reconstruction from genomic data.
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