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Genetic effects on male mouse kidney glucuronidase activity
Biochemical Genetics
|October 1, 1976
Summary
Kidney beta-glucuronidase activity varies significantly between mouse strains, with the buff coat color allele influencing enzyme levels. Genetic factors beyond the buff locus also contribute to these differences.
Area of Science:
- Genetics
- Biochemistry
- Mouse Models
Background:
- Kidney beta-glucuronidase activity exhibits strain-specific variations in mice.
- Previous studies suggest genetic influences on enzyme activity levels.
Purpose of the Study:
- To investigate the genetic basis of differing kidney beta-glucuronidase activity in mouse strains.
- To determine the association between coat color genes and enzyme activity.
Main Methods:
- Comparative analysis of kidney beta-glucuronidase activity in C57BL/K1, DBA/2/K1, and C57BL/6J male mice.
- Genetic analysis using backcross distributions to identify contributing loci.
Main Results:
- DBA/2/K1 mice showed approximately tenfold higher kidney beta-glucuronidase activity than C57BL/K1 mice.
- An association was found between kidney glucuronidase activity and the buff locus (bf allele), indicating Gur locus involvement.
- The bf allele was shown to increase enzyme activity.
Conclusions:
- Genetic variation at the Gur locus significantly influences kidney beta-glucuronidase activity.
- At least one additional locus, independent of the buff locus, contributes to the observed variations in enzyme activity.