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From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
Published on: July 4, 2016
Crystallization of the oxygen-evolving reaction centre of photosystem II in nine different detergent mixtures
1Department of Chemistry, The Technion, -Technion City, Haifa 32000, Israel. nadir@tx.technion.ac.il
Abstract:
Oxygen-evolving photosystem II reaction centres (RCII) isolated from both spinach and pea have been crystallized. A single crystal form grew from RCII monomers in the presence of nine different three-component mixtures of non-ionic detergents and heptane-1,2, 3-triol. The crystals grew as hexagonal rods with dimensions of up to 1 x 0.3 x 0.3 mm. The crystals diffracted to a maximum resolution of 6.5 A and belong to a hexagonal space group with unit-cell parameters a = 495, b = 495, c = 115 A, alpha = beta = 90, gamma = 120 degrees. The growth of a single crystal form in the presence of such a large variety of detergents suggests a very limited range of crystal lattice formation sites in the RCII complex.
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