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Crystallization and preliminary X-ray studies of beta-1, 4-galactanase from Aspergillus aculeatus
C Ryttersgaard1, J Poulsen, S Christgau
1Centre for Crystallographic Studies, H. C. Orsted Institute, University of Copenhagen, Universitetsparken 5, Copenhagen DK-2100 Denmark.
Abstract:
Recombinant beta-1,4-galactanase from Aspergillus aculeatus has been crystallized and characterized by X-ray diffraction. Crystals were obtained in hanging drops by vapour-diffusion under the conditions 30% PEG 400, 0.2 M CaCl2 and 0.1 M Na HEPES buffered to pH 7.5. The crystals diffract to 2.3 A resolution and belong to one of the orthorhombic space groups I222 or I212121. The unit-cell dimensions are a = 60.42, b = 88.94 and c = 129.08 A. With one molecule in the asymmetric unit, the corresponding solvent content is approximately 48%.