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Crystallization and preliminary X-ray data for the human transthyretin-retinol-binding protein (RBP) complex bound to
G Malpeli1, G Zanotti, F Gliubich
1Institute of Biochemical Sciences, Science Faculty, University of Parma, I-43100 Parma, Italy.
Abstract:
A macromolecular complex of human transthyretin, human retinol-binding protein and an anti-retinol-binding-protein Fab was crystallized by vapour diffusion in sitting drops. Diffraction from these crystals at cryogenic temperatures was consistent with the space group C222, with cell parameters a = 159.34, b = 222.40 and c = 121.27 A. Crystals diffracted to a resolution limit of 3.36 A using synchrotron radiation. Based on a 2:2:1 stoichiometry for the Fab-retinol-binding-protein-transthyretin complex and the presence of one such complex per asymmetric unit, a reasonable Vm coefficient of 2.74 A3 Da-1 could be estimated.