Related Experiment Video
Updated: Aug 1, 2026
Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
Published on: December 30, 2016
Expression, purification, crystallization and preliminary X-ray diffraction results from Campylobacter jejuni
S Clerté1, A Dautant, B Langlois d'Estaintot
1Unité de Biophysique Structurale (UPRES A 5471), Université de Bordeaux I, Avenue des Facultés, Bâtiment B8, 33405 Talence CEDEX, France.
Abstract:
The prokaryotic ferritin gene of Campylobacter jejuni was overexpressed in Escherichia coli under control of the bacteriophage T7 promoter and the protein (Cj-FTN) purified. Preliminary crystallization experiments have been performed using the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. Diffraction studies show the crystals belong to the I432 space group (a = 151.52 A). Structure solution by molecular replacement is in progress while crystal quality improvement is carried out.
