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Updated: Aug 19, 2026
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Nanomanipulation of Single RNA Molecules by Optical Tweezers
Published on: August 20, 2014
Preliminary crystallographic characterization of an in vitro evolved biotin-binding RNA pseudoknot
J C Nix1, A R Newhoff, C Wilson
1Department of Biology and Center for the Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA.
Abstract:
A biotin-binding RNA pseudoknot developed through in vitro selection has been crystallized using the hanging-drop vapor-phase diffusion method. The X-ray diffraction data indicate that the crystals belong to the space group P4222 with unit-cell parameters a = b = 55.2, c = 62.7 A and alpha = beta = gamma = 90 degrees. The crystals are 120 x 80 x 40 microm and VM = 2.17 A3 Da-1. The crystals diffract to 2.8 A.
