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Updated: Aug 16, 2026
Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
Preliminary characterization of crystals of an in vitro evolved cyanocobalamin (vitamin B12) binding RNA
D Sussman1, D Greensides, K Reilly
1Department of Biology and Center for the Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA.
Abstract:
A 35-nucleotide pseudoknot that binds vitamin B12 been isolated using systematic evolution of ligands by exponential enrichment (SELEX). Affinity chromatography was used to purify functional, properly folded molecules and the hanging-drop vapor-diffusion method was used to crystallize this aptamer. Two crystal forms have been obtained and the preliminary crystallographic characterization is reported here. Both crystal forms (space groups I222 or I212121 and C2221) diffract to 2.9 A and should prove sufficient for structure determination.
