Crystallization and preliminary X-ray analysis of human muscle creatine kinase

Creatine kinase is a key enzyme in the energy homeostasis of cells and tissues with high and fluctuating energy demands. Human muscle MM creatine kinase is a dimeric protein with a molecular weight of approximately 43 kDa for each subunit. It has been crystallized by the hanging-drop vapor-diffusion method using 2-methyl-2, 4-pentanediol as precipitant. The crystals belong to the enantiomorphous space group P6222 or P6422 with cell parameters of a = b = 89.11 and c = 403.97 A. The asymmetric unit of the crystal contains two subunits. A data set at 3.3 A resolution has been collected using synchrotron radiation.