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Related Experiment Videos

Structure determination of echovirus 1.

D J Filman1, M W Wien, J A Cunningham

  • 1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.

Acta Crystallographica. Section D, Biological Crystallography
|March 25, 1999
PubMed
Summary
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The atomic structure of echovirus 1 was determined using cryo-crystallography. This study highlights how crystal packing analysis aids in resolving complex symmetries for molecular replacement.

Area of Science:

  • Structural Biology
  • Virology
  • Crystallography

Background:

  • Echovirus 1 belongs to the enterovirus genus within the picornavirus family.
  • Understanding viral structures is crucial for developing antiviral strategies.

Purpose of the Study:

  • To determine the atomic structure of echovirus 1.
  • To illustrate the utility of packing analysis in molecular replacement.

Main Methods:

  • Cryo-crystallography was employed to determine the structure.
  • The structure was refined to 3.55 A resolution.
  • Noncrystallographic symmetry averaging was utilized.

Main Results:

  • The atomic structure of echovirus 1 was resolved.

Related Experiment Videos

  • Echovirus 1 exhibits specific crystallization properties (space group P22121) and pseudo-B-centering.
  • Packing analysis significantly aided in determining the virus's orientation and position.

    • Conclusions:

    • The structure determination showcases the power of packing analysis in molecular replacement.
    • Interactions between crystallographic and noncrystallographic symmetries can be resolved through advanced analysis.