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Crystallization and preliminary crystallographic study of tropinone reductase II from Datura stramonium
A Yamashita1, K Nakajima, H Kato
1Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
Abstract:
Tropinone reductase II is an NADPH-dependent oxidoreductase involved in a plant alkaloid metabolism. The enzyme from Datura stramonium has been crystallized using 2-methyl-2,4-pentanediol as a precipitant and a macro-seeding technique. The crystal belongs to space group P42212, with cell dimensions a = b = 62.8 and c = 128.4 A. A data set to 2.6 A resolution has been collected at a cryogenic temperature.