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Overexpression, purification, crystallization and preliminary X-ray diffraction analysis of the receiver domain of
M Sol1, F Gomis-Rüth, A Guasch
1Departament de Biologia Molecular i Cellular, Centre d'Investigació i Desenvolupament-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain.
Abstract:
PhoB is the response regulator of the E. coli two-component signal transduction system for phosphate regulation. It is a transcription factor that activates more than 30 genes of the pho regulon. Crystals of the receiver domain of PhoB were obtained by applying the hanging-drop vapour-diffusion method. X-ray diffraction data have been collected using synchrotron radiation to 1.88 A resolution. The crystals belong to the orthorhombic space group P212121 with unit-cell constants a = 34.11, b = 60.42, c = 119.97 A. The Matthews parameter suggests that PhoB crystallizes with two molecules per asymmetric unit, suggesting that activating dimerization occurs in the crystal.