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Related Experiment Videos

Oocyte cryopreservation.

R Fabbri1, E Porcu, T Marsella

  • 1Infertility and IVF Center, University of Bologna, Italy.

Human Reproduction (Oxford, England)
|March 26, 1999
PubMed
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Human oocyte cryopreservation shows promise, with a 60% survival rate using a slow freeze-thaw method. Optimizing cryoprotectant concentration and cumulus oophorus removal improves success for fertilization via intracytoplasmic sperm injection (ICSI).

Area of Science:

  • Reproductive biology
  • Cryobiology
  • In vitro fertilization

Background:

  • Cryopreservation of human oocytes offers a solution to legal and ethical concerns associated with embryo freezing.
  • Previous attempts at human oocyte cryopreservation have yielded limited success, characterized by low post-thaw survival rates.
  • The development of an optimal cryopreservation protocol for human oocytes remains a significant challenge in reproductive medicine.

Purpose of the Study:

  • To evaluate factors influencing human oocyte survival rates post-cryopreservation.
  • To assess the efficacy of intracytoplasmic sperm injection (ICSI) for fertilizing cryopreserved human oocytes.
  • To determine the impact of cryopreservation on embryonic morphological quality.

Main Methods:

  • A slow freeze-rapid thaw cryopreservation technique was employed using 1,2 propanediol and sucrose as cryoprotectants.

Related Experiment Videos

  • Human oocytes from 38 women with tubal infertility were cryopreserved.
  • Oocytes were cryopreserved with either partially or totally removed cumulus oophorus.

    • Main Results:

    • The overall oocyte survival rate after cryopreservation and thawing was approximately 60%.
    • Higher survival rates were observed when oocytes were cryopreserved with partially removed cumulus oophorus compared to completely removed cumulus oophorus.
    • Cryoprotectant concentration and equilibration time were identified as influential factors affecting oocyte survival rates.
    • Intracytoplasmic sperm injection (ICSI) demonstrated efficiency in achieving fertilization of cryopreserved oocytes.
    • Cryopreservation did not appear to compromise embryonic morphological quality.

    Conclusions:

    • Cryopreservation protocols, particularly those involving partial cumulus oophorus removal, can yield adequate human oocyte survival rates.
    • Intracytoplasmic sperm injection (ICSI) is an effective method for fertilizing cryopreserved human oocytes, leading to normal fertilization and embryo development.
    • Cryopreservation maintains the integrity of human oocytes, making them suitable for assisted reproductive technologies.