Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microbial degradation of polyurethane, polyester polyurethanes and polyether polyurethanes.

T Nakajima-Kambe1, Y Shigeno-Akutsu, N Nomura

  • 1Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan. toshi@sakura.cc.tsukuba.ac.jp

Applied Microbiology and Biotechnology
|March 26, 1999
PubMed
Summary

Researchers identified a unique enzyme, PUR esterase from Comamonas acidovorans TB-35, that degrades polyester polyurethane (PUR). This enzyme

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Alcanivorax borkumensis</i> biofilms enhance oil degradation by interfacial tubulation.

Science (New York, N.Y.)·2023
Same author

Growth activation of influenza virus by trypsin and effect of T-705 (favipiravir) on trypsin-optimized growth condition.

Acta virologica·2019
Same author

Kidney enlargement and multiple liver cyst formation implicate mutations in PKD1/2 in adult sporadic polycystic kidney disease.

Clinical genetics·2018
Same author

T-705 (Favipiravir) suppresses tumor necrosis factor α production in response to influenza virus infection: A beneficial feature of T-705 as an anti-influenza drug.

Acta virologica·2017
Same author

Evaluation of the Microsemi CRP, an automated hematology analyzer for rapid 3-part WBC differential and CRP using whole blood.

International journal of laboratory hematology·2014
Same author

In vitro and in vivo anticancer effects of mevalonate pathway modulation on human cancer cells.

British journal of cancer·2014

Area of Science:

  • Biochemistry
  • Polymer Science
  • Environmental Microbiology

Background:

  • Polyester polyurethane (PUR) is a widely used polymer vulnerable to microbial degradation.
  • Plastic waste pollution highlights the need for biodegradable materials like polyester PUR.
  • Microbial degradation of polyester PUR primarily involves ester bond hydrolysis by esterases.

Purpose of the Study:

  • To characterize the polyester-PUR-degrading enzyme (PUR esterase) from Comamonas acidovorans TB-35.
  • To investigate the role of the enzyme's unique structural domains in PUR degradation.
  • To explore the evolutionary relationship between PUR esterase and other polyester-degrading enzymes.

Main Methods:

  • Purification and characterization of PUR esterase from Comamonas acidovorans TB-35.

Related Experiment Videos

  • Analysis of enzyme domains, including the hydrophobic PUR-surface-binding domain.
  • Comparative sequence homology analysis with other polyester-degrading enzymes, such as PHA depolymerases.

    • Main Results:

    • PUR esterase possesses distinct hydrophobic PUR-surface-binding and catalytic domains essential for degradation.
    • The enzyme's surface-binding domain is crucial for its activity on solid polyester PUR.
    • Limited sequence homology with PHA depolymerases suggests a different evolutionary origin for PUR esterase.

    Conclusions:

    • PUR esterase represents a novel class of enzymes for degrading solid polyester polyurethane.
    • The enzyme's unique structure, particularly the binding domain, is key to its function.
    • PUR esterases may warrant classification as a new family of solid-polyester-degrading enzymes.