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Related Experiment Videos

A murC gene from coryneform bacteria.

M Wachi1, C D Wijayarathna, H Teraoka

  • 1Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan. mwachi@bio.titech.ac.jp

Applied Microbiology and Biotechnology
|March 26, 1999
PubMed
Summary
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Brevibacterium flavum MJ233 possesses the murC gene upstream of ftsQ, crucial for peptidoglycan synthesis and cell division. This finding reveals a conserved gene cluster for cell wall synthesis and division in coryneform bacteria.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Brevibacterium flavum MJ233 is a coryneform bacterium.
  • Cell wall synthesis and cell division are essential processes in bacteria.
  • The organization of genes involved in these processes can vary between species.

Purpose of the Study:

  • To identify genes involved in cell wall synthesis in B. flavum.
  • To investigate the genetic organization of cell division and cell wall synthesis genes in B. flavum.
  • To compare the gene organization with that of Escherichia coli.

Main Methods:

  • Inverse polymerase chain reaction (PCR) for gene amplification.
  • Cloning of amplified DNA fragments in Escherichia coli.
  • Complementation analysis of E. coli mutants.

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  • DNA sequencing.
  • In vitro transcription/translation assays.

    • Main Results:

    • The murC gene, encoding UDP-N-acetylmuramate:L-alanine ligase, was identified upstream of the ftsQ gene in B. flavum.
    • The B. flavum murC gene encodes a 486-amino acid protein with a molecular mass of 51,198 Da.
    • A 50-kDa protein was synthesized in vitro, confirming gene expression.
    • Genes for cell wall synthesis and cell division appear to be clustered in B. flavum.

    Conclusions:

    • The murC gene is essential for peptidoglycan synthesis in B. flavum.
    • Cell wall synthesis and cell division genes are organized in a cluster in B. flavum, similar to the E. coli mra region.
    • This conserved gene organization suggests functional similarities between B. flavum and E. coli.