Related Experiment Video
Updated: Aug 13, 2026
Establishment of a Simple and Effective Rat Model for Intraoperative Parathyroid Gland Imaging
Published on: August 17, 2022
Enhanced visualization of parathyroid tissue by infusion of a visible dye conjugated to an antiparathyroid antibody
R C King1, S L Mills, J E Medina
1Department of Medicinal Chemistry and Pharmaceutics, College of Pharmacy, The University of Oklahoma Health Sciences Center, Oklahoma City, USA.
Background:
The antiparathyroid antibody BB5-G1 conjugated to cibacron blue, a blue dye, was intravenously infused to enhance parathyroid visualization. Previously, we demonstrated selective staining of human parathyroid implants in athymic nude mice after infusion of the conjugate.
Methods:
Mice possessing implanted parathyroid tissue were randomized into the following: group I, infused with cibacron blue alone; group II, infused with the antibody/dye conjugate; and group III, infused with radiolabeled BB5-G1 alone. Implants were surgically explored. Three blinded observers ranked parathyroid visualization in the operative fields, and corresponding histologic sections were computer analyzed.
Results:
Group II implants were easily visualized; groups I and III showed no staining. Group III showed high gamma counts. Subjective rankings and computer rankings correlated well (p < .05). Group II showed a higher mean staining intensity of 27.45 picogram protein product (PPP)/cell compared with 7.76 PPP/cell of group I (p = .002).
Conclusion:
Cibacron blue/BB5-G1 consistently enhances visualization of implanted parathyroid tissue.
