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Infection of apheresis cells by parvovirus B19
Abstract:
Parvovirus B19 is the only member of the Parvoviridae family known to cause disease in humans. Owing to the high level of cell tropism the virus can only replicate in proliferating and differentiating erythroid precursor cells, which are present in human bone marrow and foetal liver. As human bone marrow is very difficult to obtain, an alternative in vitro system for the propagation of B19 virus has been developed, based on the application of mobilized haemapoietic progenitor (apheresis) cells. These cells are routinely harvested from cancer patients after treatment with recombinant human granulocyte/macrophage colony-stimulating factor. Replication of parvovirus B19 in vitro is possible in these cells after stimulation with erythropoietin. Therefore, this system is an easily, accessible alternative to the use of human bone marrow in parvovirus B19 infection assays.
Insights
A new method uses apheresis cells to grow Parvovirus B19 in vitro. This provides an accessible alternative to human bone marrow for studying Parvovirus B19 infections.
Area of Science:
- Virology
- Hematology
Background:
- Parvovirus B19, a human pathogen, exclusively replicates in erythroid precursor cells.
- Human bone marrow, a primary source for these cells, is difficult to obtain for research.
- Existing methods for Parvovirus B19 propagation are limited.
Purpose of the Study:
- To establish an accessible in vitro system for Parvovirus B19 propagation.
- To develop an alternative to human bone marrow for Parvovirus B19 research.
Main Methods:
- Utilized mobilized hematopoietic progenitor (apheresis) cells from cancer patients treated with granulocyte/macrophage colony-stimulating factor.
- Stimulated erythroid precursor cell replication in vitro using erythropoietin.
- Cultured Parvovirus B19 in the prepared apheresis cell system.
Main Results:
- Successfully propagated Parvovirus B19 in vitro using apheresis cells.
- Demonstrated that erythropoietin is crucial for viral replication in this system.
- Established a reliable method for obtaining sufficient viral material for assays.
Conclusions:
- Mobilized hematopoietic progenitor cells offer a viable and accessible alternative to human bone marrow for Parvovirus B19 propagation.
- This in vitro system facilitates further research into Parvovirus B19 pathogenesis and antiviral strategies.
- The findings simplify experimental approaches to studying Parvovirus B19 infections.