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Structure of two maize phytase genes and their spatio-temporal expression during seedling development
S Maugenest1, I Martinez, B Godin
1Laboratoire de Biologie des Semences, INRA INA-PG, Versailles, France.
Plant Molecular Biology
|March 26, 1999
Summary
Two maize phytase genes, PHYT I and PHYT II, are identified. Both genes are expressed during germination, but only PHYT I is active in adult plant roots, indicating distinct regulatory signals.
Area of Science:
- Plant Molecular Biology
- Biochemistry
Background:
- Phosphorus in Zea mays (corn) grain is primarily stored as phytin in the embryo.
- Phytin mobilization is crucial for phosphorus supply during germination and seedling development.
Purpose of the Study:
- To investigate the control of phytin mobilization during maize germination and seedling growth.
- To isolate and characterize genes encoding phytase in Zea mays.
Main Methods:
- Screening of a maize genomic library using a phytase cDNA (phy S11).
- Isolation and sequencing of two distinct phytase genes (PHYT I and PHYT II).
- Northern blot, in situ hybridization, immunolocalization, and RT-PCR analyses to study gene expression patterns.
Main Results:
- Two homologous phytase genes, PHYT I and PHYT II, were identified with distinct non-transcribed regions.
- Phytase mRNA and protein accumulate in the coleorhiza, radicle cortex, coleoptile parenchyma, and scutellum during germination.
- Phytase mRNA and protein are detected in adult plant roots (rhizodermis, endodermis, pericycle).
- Both PHYT I and PHYT II are expressed during germination, while only PHYT I is expressed in adult roots.
Conclusions:
- Maize possesses at least two phytase genes with differential expression patterns.
- Distinct regulatory mechanisms control phytase gene expression during germination and in adult roots.
- Understanding these genes is key to improving phosphorus utilization in maize.