Related Experiment Videos
Differentially expressed genes in activin-induced apoptotic LNCaP cells
Biochemical and Biophysical Research Communications
|March 27, 1999
Summary
Researchers identified gene expression changes in human prostate cancer cells undergoing apoptosis. A novel kinase-like gene was found to be crucial for DNA fragmentation during activin-induced apoptosis.
Area of Science:
- Molecular Biology
- Cancer Research
- Cell Biology
Background:
- Activin induces cell-cycle arrest and apoptosis in human prostatic LNCaP cells.
- Understanding gene expression changes during apoptosis is crucial for cancer research.
Purpose of the Study:
- To identify differentially expressed genes in activin-induced LNCaP apoptotic cells.
- To elucidate the molecular mechanisms underlying activin-induced apoptosis.
Main Methods:
- Utilized an improved subtractive hybridization method, the uracil-DNA subtraction assay (USA).
- USA involves digestion with uracil-DNA glycosylase and mung-bean nuclease.
- Performed anti-sense knock-out of a novel kinase-like gene.
Main Results:
- Identified 5 up-regulated and 7 down-regulated genes.
- Discovered 6 known genes (p16, p53, Siva, RHAMM, Pax2, eIF-4a1), 3 homologues, and 3 novel genes.
- Anti-sense knock-out of a novel kinase-like gene abolished apoptotic DNA fragmentation.
Conclusions:
- A novel kinase-like gene plays a critical role in DNA fragmentation during activin-induced apoptosis.
- Findings suggest a new mechanism in DNA fragmentation of activin-induced cell-cycle arresting and apoptosis.