Related Experiment Videos
Novel substrates of yeast alcohol dehydrogenase--4. Allyl alcohol and ethylene glycol
Biochemistry and Molecular Biology International
|March 27, 1999
Abstract:
In the present work, we have determined the steady-state kinetic constants for yeast alcohol dehydrogenase-catalyzed oxidation of allyl alcohol (H2C = CH.CH2OH) and ethylene glycol (HOCH2.CH2OH) with NAD+, at pH 8.0; also, a kinetic mechanism for the former reaction was determined at the same pH. In addition, it was found that acrolein is a potent inhibitor of yeast alcohol dehydrogenase.