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Lymph node revealing solution: a rapid method for the fixation of cystectomy specimens
R Koren1, A Paz, M Konichezsky
1Department of Pathology, Beilinson Hospital, Rabin Medical Center, Petah Tikva, Israel.
Pathology, Research and Practice
|March 27, 1999
Summary
Lymph node revealing solution (LNRS) offers rapid, effective fixation for cystectomy specimens. This method provides excellent tissue preservation and staining quality in just two hours, outperforming traditional formalin fixation.
Area of Science:
- Surgical Pathology
- Histotechnology
- Uropathology
Background:
- Traditional formalin fixation of cystectomy specimens is time-consuming.
- Optimizing fixation methods is crucial for timely and accurate histopathological diagnosis.
Purpose of the Study:
- To evaluate the efficacy of lymph node revealing solution (LNRS) for rapid fixation of total cystectomy specimens.
- To compare LNRS fixation with conventional buffered formalin fixation.
Main Methods:
- Sixteen cystectomy specimens were fixed for two hours using either LNRS or buffered formalin.
- Tissues were processed, embedded in paraffin, sectioned, and stained using H&E and various immunohistochemical markers.
Main Results:
- LNRS provided excellent tissue fixation within two hours, while formalin fixation was inadequate.
- Paraffin block processing and sectioning were superior with LNRS fixation.
- All histological and immunohistochemical stains demonstrated excellent quality with LNRS fixation.
Conclusions:
- LNRS is a highly effective and rapid method for cystectomy specimen fixation.
- The two-hour LNRS fixation protocol yields superior tissue preservation and staining results compared to short-term formalin fixation.
- LNRS is recommended for routine fixation of cystectomy specimens.