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Isolation of an active catalytic core of Streptococcus downei MFe28 GTF-I glucosyltransferase
V Monchois1, M Arguello-Morales, R R Russell
1Department of Oral Biology, The Dental School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4BW, United Kingdom. vincent.monchois@ncl.ac.uk
Journal of Bacteriology
|March 27, 1999
Abstract:
Truncated variants of GTF-I from Streptococcus downei MFe28 were purified by means of a histidine tag. Sequential deletions showed that the C-terminal domain was not directly involved in the catalytic process but was required for primer activation. A fully active catalytic core of only 100 kDa was isolated.