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Related Experiment Videos

Urinary nucleosides.

K H Schram1

  • 1Department of Pharmacology and Toxicology, College of Pharmacy, University of Arizona, Tucson 85721, USA.

Mass Spectrometry Reviews
|March 30, 1999
PubMed
Summary

Urinary nucleosides show potential as biomarkers for diseases like cancer. However, current analytical methods require further refinement for reliable clinical decision-making based on nucleoside levels.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Urinary nucleosides are compounds found in human urine, with 57 identified in both healthy and diseased states.
  • Their origins and clinical significance have been studied, particularly in relation to various diseases.
  • Previous research has explored their potential as indicators of health status.

Purpose of the Study:

  • To review the analytical methods, origins, and clinical significance of urinary nucleosides up to 1997.
  • To present structural, chromatographic, and mass spectral data for identified urinary nucleosides.
  • To compare the advantages and limitations of different analytical techniques for urinary nucleoside analysis.

Main Methods:

  • Gas Chromatography (GC)
  • High-Performance Liquid Chromatography (HPLC)
  • Gas Chromatography-Mass Spectrometry (GC/MS)
  • High-Performance Liquid Chromatography-Mass Spectrometry (HPLC/MS)
  • Immunoassays
  • Sample preparation techniques

Main Results:

  • Detailed data for 57 urinary nucleosides, including structures and spectral information, are presented.
  • Analysis of 37 individual HPLC fractions using HPLC separation and GC/MS is discussed.
  • A comparison of analytical methods highlights their respective strengths and weaknesses.

Conclusions:

  • Urinary nucleosides are confirmed as potential biomarkers for cancer and other diseases.
  • Analytical methodologies for urinary nucleoside detection need enhancement for clinical application.
  • Further improvements in analytical techniques are necessary for reliable clinical decisions based on urinary nucleoside levels.

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