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Growth medium affects the cellular fatty acid composition of Pasteurellaceae
R Boot1, H C Thuis, F A Reubsaet
1Section of Laboratory Animal Microbiology, National Institute of Public Health and the Environment, Bilthoven, The Netherlands. r.boot@rivm.nl
Zentralblatt Fur Bakteriologie : International Journal of Medical Microbiology
|March 30, 1999
Summary
Cellular fatty acid profiling of Pasteurellaceae using the MIDI Microbial Identification System (MIS) is sensitive to growth media. Optimal discrimination of species was achieved using VH agar, highlighting the importance of standardized conditions for accurate identification.
Area of Science:
- Microbiology
- Analytical Chemistry
- Bacterial Taxonomy
Background:
- Cellular fatty acid profiles are crucial for bacterial identification.
- The MIDI Microbial Identification System (MIS) is widely used for this purpose.
- Standardization of growth conditions is essential for reproducible results.
Purpose of the Study:
- To investigate the effect of two different agar media on the cellular fatty acid composition of Actinobacillus and Pasteurella species.
- To determine if principal component analysis (PCA) of fatty acid methyl ester (FAME) profiles can differentiate these species based on growth medium.
Main Methods:
- Ten reference strains of Actinobacillus and Pasteurella were grown on Levinthal's agar (LA) and VH agar.
- Cellular fatty acid profiles were analyzed using the MIDI Microbial Identification System (MIS).
- Fatty acid methyl ester (FAME) profiles were subjected to principal component analysis (PCA).
Main Results:
- All Pasteurellaceae strains exhibited FAME profiles characteristic of the family.
- Growth on VH agar resulted in lower coefficients of variation for major FAMEs compared to LA agar, indicating better reproducibility.
- PCA of FAME profiles from VH agar allowed for clear discrimination of most Pasteurellaceae species, whereas LA agar did not.
Conclusions:
- The growth medium significantly impacts the reproducibility of fatty acid profiling in Pasteurellaceae.
- Standardized growth conditions, particularly using VH agar, are critical for accurate species discrimination using PCA of FAME data.
- This study provides a foundation for improved identification of Pasteurellaceae species through optimized fatty acid analysis.