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Related Experiment Videos

Increased typability of multiresistant Staphylococcus aureus by reverse phage typing.

M Ben-Yaakov1, Z Lazarovich, N Boldur

  • 1Department of Microbiology, Assaf Harofeh Medical Center, Zerifin, Israel.

Zentralblatt Fur Bakteriologie : International Journal of Medical Microbiology
|March 30, 1999
PubMed
Summary

Reverse typing significantly improves Staphylococcus aureus typing, especially for multiresistant strains, enhancing antimicrobial resistance surveillance in hospitals. This method is crucial for identifying and tracking challenging infections.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Clinical Diagnostics

Background:

  • Conventional phage typing methods struggle to classify a significant portion of Staphylococcus aureus isolates.
  • Antimicrobial resistance, particularly to penicillin G and oxacillin, is prevalent in hospital-acquired S. aureus.
  • Understanding isolate characteristics is vital for effective infection control and treatment.

Purpose of the Study:

  • To evaluate the efficacy of reverse typing as a supplementary method to conventional phage typing for Staphylococcus aureus isolates.
  • To characterize antibiotic resistance patterns and identify distinct groups of oxacillin-resistant S. aureus.
  • To determine the utility of reverse typing for multiresistant S. aureus strains.

Main Methods:

  • Conventional phage typing was performed on Staphylococcus aureus isolates.

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  • Reverse typing was employed as an additional or alternative typing method.
  • Antibiotic susceptibility testing was conducted for penicillin G, oxacillin, and other common antibiotics.

    • Main Results:

    • Conventional phage typing identified only 60% of isolates; reverse typing increased typability to 75%.
    • Reverse typing significantly improved the typability of antibiotic-resistant isolates to over 80%.
    • Two main groups of oxacillin-resistant isolates were identified: partially resistant (typable by conventional methods) and multiresistant (typable by reverse typing).

    Conclusions:

    • Reverse typing is a valuable tool for enhancing the characterization of Staphylococcus aureus, particularly multiresistant strains.
    • The study highlights the emergence and persistence of multiresistant S. aureus in hospital settings.
    • Reverse typing offers improved diagnostic capabilities for epidemiological surveillance of S. aureus infections.