Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thrombogenic stimulus causes long-term decrease of muscle flap perfusion.

B D Krapohl1, M Siemionow, P Piza

  • 1Orthopädische Klinik Kassel, Germany.

Annals of Plastic Surgery
|March 30, 1999
PubMed
Summary

A continuous thrombogenic stimulus in the feeding artery significantly reduced skeletal muscle flap capillary perfusion over 24 hours, despite maintained blood flow. This highlights the impact of arterial insults on microcirculation in muscle flaps.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Urinary tract infection's etiopathogenic role in nephrolithiasis formation.

Medical hypotheses·2018
Same author

Creation of Dystrophin Expressing Chimeric Cells of Myoblast Origin as a Novel Stem Cell Based Therapy for Duchenne Muscular Dystrophy.

Stem cell reviews and reports·2018
Same author

Comparative analysis between percutaneous nephrolithotomy and flexible ureteroscopy in kidney stones of 2-3cm.

Actas urologicas espanolas·2016
Same author

Establishing the Feasibility of Face Transplantation in Granulomatosis With Polyangiitis.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2016
Same author

Novel approach to treat fecal incontinence with muscle stem cell-based therapy.

Techniques in coloproctology·2015
Same author

Has consultation achieved its primary prevention potential?

The journal of primary prevention·2013

Area of Science:

  • Vascular Surgery
  • Microcirculation Research
  • Tissue Engineering

Background:

  • Maintaining adequate perfusion is critical for skeletal muscle flap viability.
  • Thrombogenic insults to feeding arteries can compromise flap survival.
  • Understanding the microcirculatory effects of arterial thrombosis is essential for surgical outcomes.

Purpose of the Study:

  • To evaluate the impact of a continuous thrombogenic insult on skeletal muscle flap perfusion over 24 hours.
  • To investigate the microcirculatory changes in response to an induced arterial thrombus.
  • To assess the relationship between feeding artery thrombosis and capillary perfusion in muscle flaps.

Main Methods:

  • Induction of a continuous thrombogenic stimulus in the common iliac artery of Sprague-Dawley rats using an inverting suture.

Related Experiment Videos

  • Isolation and preservation of the cremaster muscle flap on its neurovascular pedicle for 24-hour observation.
  • Intravital microcirculatory measurements including red blood cell velocity, vessel diameter, capillary perfusion, endothelial edema, and leukocyte-endothelial interactions.

    • Main Results:

    • No significant differences were observed in red blood cell velocities, vessel diameters, or leukocytic-endothelial interactions between control and treatment groups.
    • A significant decrease in capillary perfusion was observed in the treatment group, dropping from 6.23 to 1.50 capillaries per visual field (p = 0.002).
    • The thrombogenic insult significantly impaired capillary perfusion despite maintained blood flow in the main feeding artery.

    Conclusions:

    • A continuous arterial thrombogenic insult can lead to a substantial reduction in skeletal muscle flap capillary perfusion.
    • Microcirculatory impairment, specifically decreased capillary perfusion, is a key consequence of feeding artery thrombosis in muscle flaps.
    • These findings underscore the importance of preventing or managing arterial thrombosis to ensure successful skeletal muscle flap survival and function.