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Updated: Aug 14, 2026

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Published on: August 6, 2011
Use of combined frequencies for RFLP and PCR based loci in determining match probability
C S Tomsey1, C J Basten, B Budowle
1Pennsylvania State Police Bureau of Forensics and Criminal Identification, Forensics Division, Greensburg 15601, USA.
Abstract:
Statistical analysis was performed on a subset of the Pennsylvania State Police Caucasian, African American and Hispanic database for the purpose of determining Hardy-Weinberg equilibrium and associations across the RFLP loci D1S7, D2S44, D4S139, D5S110, D10S28 and D17S79 and the PCR-based loci HLA-DQA1, LDLR, GYPA, HBGG, D7S8 and Gc. Overall, the statistical results are consistent with a population in equilibrium both within and between loci. The assumption for independence is valid.
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