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Related Experiment Videos

Age-associated change in mitochondrial DNA damage.

E K Hudson1, B A Hogue, N C Souza-Pinto

  • 1Laboratory of Molecular Genetics, Gerontology Research Center, NIA, NIH, Baltimore, MD 21224-6823, USA.

Free Radical Research
|March 31, 1999
PubMed
Summary

Aging Wistar rats show increased mitochondrial DNA damage, specifically 8-oxo-2'-deoxyguanosine (8-oxodG), which may explain age-related declines in heart mitochondrial function.

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DNA Polymerase Beta Participates in Mitochondrial DNA Repair.

Molecular and cellular biology·2017

Area of Science:

  • Gerontology
  • Molecular Biology
  • Cardiovascular Research

Background:

  • Mitochondrial dysfunction is linked to aging.
  • Previous studies show decreased cytochrome c oxidase (COX) activity and protein synthesis in aging rat hearts.
  • Mitochondrial DNA (mtDNA) damage is a potential contributor to this decline.

Purpose of the Study:

  • To investigate age-associated changes in oxidative damage to nuclear and mitochondrial DNA in Wistar rat liver.
  • To quantify levels of 8-oxo-2 -deoxyguanosine (8-oxodG), a marker of oxidative DNA damage, in young and senescent rats.

Main Methods:

  • HPLC-Coularray system used to measure 8-oxodG in nuclear and mitochondrial DNA from 6-month (young) and 23-month-old (senescent) Wistar rats.
  • Calf thymus DNA was damaged to validate technique sensitivity.

Related Experiment Videos

  • 8-oxodG levels reported as a fraction of 2-deoxyguanosine (2-dG).

    • Main Results:

    • No significant change in nuclear DNA 8-oxodG levels with age.
    • A 2.5-fold increase in mitochondrial DNA 8-oxodG levels in senescent rats compared to young rats.
    • Mitochondrial 8-oxodG was significantly higher than nuclear 8-oxodG, increasing from 5-fold to 12-fold with age.

    Conclusions:

    • Age-associated oxidative damage to mitochondrial DNA increases significantly in Wistar rat liver.
    • This elevated mtDNA damage may contribute to the decline in mitochondrial function observed with aging.
    • Findings support the role of mtDNA integrity in the aging process and cardiovascular health.