The properties and function of invertebrate new muscle protein

We found out a new protein from natural actomyosin prepared from adductor muscle of Hokki clam, bivalve shell. We isolated this protein and determined some properties. It has a large molecular weight (230 kDa) and the star diagram of amino acid composition was very similar to that of paramyosin (110 kDa). When this protein was added to Hokki clam myosin, the Mg2+ -ATPase activity was more activated in the presence of 10(-7) M Ca2+ and further inhibited in the presence of 10(-4) M Ca2+ as compared with those of myosin. From these results, we suggest that Hokki clam adductor muscle contains another myosin-linked regulatory protein, myonin, which is different from the myosin-linked system, the myosin light chain-linked system. We named this protein 'myonin'.