Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microfabricated immunoisolating biocapsules.

T A Desai1, W H Chu, J K Tu

  • 1Biomedical Microdevices Center, University of California, Berkeley, California 94720, USA.

Biotechnology and Bioengineering
|April 1, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Key transcription factors in myeloid hematopoiesis].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2019
Same author

Meta-analysis of gene expression in human pancreatic islets after in vitro expansion.

Physiological genomics·2009
Same author

Global gene expression profiling and histochemical analysis of the developing human fetal pancreas.

Diabetologia·2007
Same author

Filtration of sub-100 nm particles using a bulk-micromachined, direct-bonded silicon filter.

Biomedical microdevices·2005
Same author

Keratinocyte growth factor and beta-cell differentiation in human fetal pancreatic endocrine precursor cells.

Diabetologia·2003
Same author

Growth and genetic modification of human beta-cells and beta-cell precursors.

Genetic engineering·2001
Same journal

Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture.

Biotechnology and bioengineering·2026
Same journal

LLM-Guided Parameter Optimization for Mechanistic CHO Cell Bioreactor Models.

Biotechnology and bioengineering·2026
Same journal

Three-Dimensional-Printed Polylactic Acid Scaffolds Coated With a Paeonol-Incorporated Gelatin/Bioactive Glass Composite Layer for Enhanced Osteogenic Performance.

Biotechnology and bioengineering·2026
Same journal

Recent Progress in Antimicrobial Peptides (AMPs) Towards Enhanced Selectivity and Reduced Cytotoxicity by Molecular Engineering.

Biotechnology and bioengineering·2026
Same journal

mZVI-Enhanced Mixed Nitrogen Removal in Klebsiella oxytoca via Coordinated Electron Transfer and Metabolic Reprogramming.

Biotechnology and bioengineering·2026
Same journal

Growth Model for Continuous Culture of a Hydrogen-Oxidizing Bacterium, Hydrogenophilus thermoluteolus Strain TH-1.

Biotechnology and bioengineering·2026
See all related articles

This study presents a silicon biocapsule for cell transplants, using microfabrication to create pores that block immune molecules. Encapsulated pancreatic islets showed improved survival and insulin secretion in vitro.

Area of Science:

  • Biomaterials Science
  • Bioengineering
  • Transplantation Immunology

Background:

  • Cell transplantation faces challenges from immune rejection and graft failure.
  • Immunoisolation strategies aim to protect transplanted cells from the host immune system.
  • Microfabrication offers precise control over device architecture for biological applications.

Purpose of the Study:

  • To develop and evaluate a microfabricated silicon-based biocapsule for immunoisolation of cell transplants.
  • To assess the biocompatibility and efficacy of encapsulated pancreatic islets for insulin delivery.

Main Methods:

  • Utilized bulk and surface micromachining of single crystalline silicon wafers to create biocapsules.
  • Engineered polycrystalline silicon filter membranes with uniform pores (as small as 20 nm) for controlled permeability.

Related Experiment Videos

  • Investigated in vitro biocompatibility and insulin secretion of encapsulated rat neonatal pancreatic islets.

    • Main Results:

    • Demonstrated successful fabrication of silicon biocapsules with high-density, nanoporous filter membranes.
    • Confirmed sufficient permeability for oxygen, glucose, and insulin, while impeding immune molecules and viruses.
    • Showcased significantly enhanced survival and insulin secretion of encapsulated pancreatic islets compared to controls over several weeks.

    Conclusions:

    • Microfabricated silicon biocapsules show promise for the immunoisolation of cell transplants.
    • The developed nanotechnology supports long-term function of encapsulated pancreatic islets, suggesting potential for diabetes treatment.
    • Further investigation into cell xenografts within these microfabricated immunoisolating environments is warranted.