Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzyme array-amperometric detection in carbohydrate analysis.

M Sun1, C S Lee

  • 1Department of Chemistry and Ames Laboratory, USDOE, Iowa State University, Ames, Iowa 50011, USA.

Biotechnology and Bioengineering
|April 1, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heme oxygenase-mediated resistance to oxygen toxicity in hamster fibroblasts.

The Journal of biological chemistry·1997
Same author

Novel and frequent mutations of hepatitis B virus coincide with a major histocompatibility complex class I-restricted T-cell epitope of the surface antigen.

Journal of virology·1997
Same author

Ras p21 protein immunoreactivity and its relationship to p53 expression and prognosis in gallbladder and extrahepatic biliary carcinoma.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·1997
Same author

On-line micellar electrokinetic chromatography-electrospray ionization mass spectrometry using anodically migrating micelles.

Analytical chemistry·1997
Same author

Lamotrigine trial in idiopathic parkinsonism: a double-blind, placebo-controlled, crossover study.

Neurology·1997
Same author

Involution of the sheep mammary gland.

Journal of anatomy·1997

This study introduces a novel enzyme array-electrochemical detection method for carbohydrate analysis. This technique directly quantifies released monosaccharides, enabling oligosaccharide structural characterization without separation or labeling.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Carbohydrate Chemistry

Background:

  • Oligosaccharide structural characterization is crucial in various biological and chemical fields.
  • Existing methods often require complex separation or labeling procedures.
  • Enzyme-based approaches offer specificity but require robust detection.

Purpose of the Study:

  • To demonstrate an enzyme array-electrochemical detection method for oligosaccharide analysis.
  • To enable direct quantification of released monosaccharides for structural elucidation.
  • To establish a label-free and separation-free analytical approach.

Main Methods:

  • Utilized an enzyme array coupled with pulsed amperometric detection at a gold electrode.
  • Analyzed N-linked oligosaccharides, including complex and high-mannose types.

Related Experiment Videos

  • Quantified released monosaccharides after enzymatic digestion.

    • Main Results:

    • Successfully demonstrated direct quantification of monosaccharides.
    • Obtained structural characterization of oligosaccharides using enzyme array data and monosaccharide concentrations.
    • The method proved effective for specific oligosaccharide samples.

    Conclusions:

    • The enzyme array-electrochemical detection method provides a direct and efficient approach for oligosaccharide structural analysis.
    • This technique eliminates the need for prior separation or labeling of carbohydrate samples.
    • Future advancements depend on developing more diverse and specific exoglycosidase arrays.