On-line estimation of biomass through pH control analysis in aerobic yeast fermentation systems
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Updated: Oct 24, 2026

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
Published on: January 23, 2019
A Vicente1, J I Castrillo, J A Teixeira
1Departamento de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, 4709 Braga codex, Portugal.
Abstract:
The amount of acid or base consumed in yeast cultures has been recently assigned to the pathway of nitrogen assimilation under respiratory conditions with no contribution by carbon metabolism (Castrillo et al., 1995). In this investigation, experiments under respirofermentative conditions have shown that production or consumption of ethanol does not contribute significantly to the specific rate of proton production (qH+), thus extending the previously obtained relationships for all aerobic conditions in which other major acid/base contributions are not involved. Tests in batch and chemostat culture confirm the validity of qH+ as a formal control parameter in aerobic fermentations.
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