Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A method of graphically analyzing substrate-inhibition kinetics.

Wang1, Araki, Ogawa

  • 1Shiga Technology Center, Iwatani International Corporation, Moriyama, 524 Japan.

Biotechnology and Bioengineering
|April 1, 1999
PubMed
Summary

A new linear-plot method simplifies the analysis of substrate inhibition kinetics in enzyme reactions and biological systems. This approach offers a visual validation of kinetic models and parameter estimation, complementing existing methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distribution of newly formed vessels in human colorectal carcinomas with microangiography.

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2013
Same author

Pharmacological profile and clinical efficacy of minodronic acid hydrate as a new therapeutic agent for osteoporosis.

Nihon yakurigaku zasshi. Folia pharmacologica Japonica·2009
Same author

Effectiveness of the portable ultrasound bladder scanner in the measurement of residual urine volume after total mesorectal extirpation.

Minimally invasive therapy & allied technologies : MITAT : official journal of the Society for Minimally Invasive Therapy·2006
Same author

The AllHear Cochlear Implant and Tinnitus.

The international tinnitus journal·2001
Same author

Regulation kinetics of Na+-Ca2+ exchange current in guinea-pig ventricular myocytes.

The Journal of physiology·2000
Same author

Preparation of high activity whole cell biocatalyst by permeabilization of recombinant flocculent yeast with alcohol.

Enzyme and microbial technology·2000

Area of Science:

  • Biochemistry
  • Biotechnology
  • Chemical Kinetics

Background:

  • Substrate inhibition is a common phenomenon in enzyme catalysis.
  • Recombinant cyanobacteria exhibit complex kinetic behaviors, such as light inhibition.
  • Analyzing these kinetics often requires advanced computational methods.

Purpose of the Study:

  • To extend a substrate inhibition model to photosynthetic ethylene production in cyanobacteria.
  • To develop and validate a linear-plot approach for analyzing inhibition kinetics.
  • To demonstrate the applicability of the linear-plot method across diverse biological systems.

Main Methods:

  • Mathematical modeling of enzyme kinetics and photosynthetic production.
  • Transformation of kinetic equations into linear forms for graphical analysis.

Related Experiment Videos

  • Application of the linear-plot method to enzyme inhibition and bacterial growth.
  • Comparison of parameter estimates with nonlinear least-squares fitting.

    • Main Results:

    • The linear-plot approach demonstrated reasonable linearity for cyanobacterial ethylene production.
    • Parameter values were successfully estimated from the linear plots.
    • The method showed good linearity and applicability to enzyme substrate inhibition and bacterial growth.
    • Visual validation of assumed kinetics was achieved for multiple systems.

    Conclusions:

    • The linear-plot approach provides a complementary and visually intuitive method for analyzing substrate inhibition kinetics.
    • This method simplifies parameter estimation and kinetic model validation.
    • It is applicable to a range of biological systems beyond enzyme catalysis.