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Modelling of oligosaccharide synthesis by dextransucrase
B Demuth1, H J Jördening, K Buchholz
1Lehrstuhl für Technologie der Kohlenhydrate, Technical University of Braunschweig, Langer Kamp 5, D-38106 Braunschweig, Germany.
Biotechnology and Bioengineering
|April 1, 1999
Summary
Dextransucrase enzyme kinetics were modeled to optimize leucrose production. Immobilized enzyme systems show promise for industrial-scale synthesis of this valuable disaccharide.
Area of Science:
- Enzymology
- Biochemical Engineering
- Carbohydrate Chemistry
Background:
- Dextransucrase enzyme synthesizes dextran and various oligosaccharides from sucrose.
- Understanding enzyme kinetics is crucial for controlling product formation.
- Leucrose, a disaccharide, is of significant industrial interest.
Purpose of the Study:
- To develop a mathematical model describing dextransucrase complex kinetic behavior.
- To investigate kinetic parameters using specific acceptors like maltose and fructose.
- To inform the design of a continuous reactor for leucrose production.
Main Methods:
- Extensive experimental kinetic tests were performed.
- A reaction scheme and mathematical model were developed.
- A computer program calculated model parameters from experimental data.
Main Results:
- The study elucidated the complex kinetic behavior of dextransucrase.
- Kinetic parameters were determined using maltose and fructose as acceptors.
- The findings provide a basis for reactor design.
Conclusions:
- The developed kinetic model accurately describes dextransucrase activity.
- Optimized conditions and modeling facilitate leucrose production.
- Immobilized dextransucrase in a continuous reactor system is a viable strategy for industrial leucrose synthesis.