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Related Experiment Videos

Electrogenic Na+/HCO3- cotransporters: cloning and physiology.

M F Romero1, W F Boron

  • 1Department of Physiology and Biophysics and Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4790, USA. mfr2@po.cwru.edu

Annual Review of Physiology
|April 1, 1999
PubMed
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The cloning of the Na+ bicarbonate cotransporter (NBC) reveals a new family of HCO3- transporters. This discovery impacts our understanding of pH buffering and transport in various organs.

Area of Science:

  • Physiology
  • Molecular Biology
  • Biochemistry

Background:

  • Bicarbonate (HCO3-) and CO2 are primary biological pH buffers.
  • Renal proximal tubule reabsorbs most filtered HCO3- via a basolateral Na+/HCO3- cotransporter (NBC).

Purpose of the Study:

  • To review recent developments concerning the cloning and characterization of the Na+ bicarbonate cotransporter (NBC).
  • To explore the physiological properties, isoforms, gene expression, and tissue distribution of NBC.

Main Methods:

  • Expression cloning of the NBC.
  • Analysis of protein features and physiological characteristics.
  • Investigation of mRNA and protein distribution across various tissues.

Main Results:

Related Experiment Videos

  • The Na+ bicarbonate cotransporter (NBC) has been cloned, revealing a 30-35% amino acid sequence identity with anion exchangers (AE1-3).
  • NBC functions electrogenically, is dependent on Na+ and HCO3-, independent of Cl-, and inhibited by stilbenes (DIDS, SITS).
  • NBC has been identified in multiple tissues beyond the kidney, including pancreas, prostate, brain, heart, and gastrointestinal tract, suggesting diverse physiological roles.

Conclusions:

  • The cloning of NBC supports the emergence of a superfamily of HCO3- transporters.
  • At least two genes encode NBC proteins, highlighting complexity in HCO3- transport regulation.
  • Further research into NBC's distinct physiological roles in various tissues is warranted.