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Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)
1Department of Chemistry and Center for Molecular Catalysis, Seoul National University, Seoul 151-742, South Korea.
Biochimica Et Biophysica Acta
|April 2, 1999
Abstract:
We cloned and expressed a full-length cDNA encoding a phospholipase D of type alpha (PLDalpha) from cabbage. Analysis of the cDNA predicted an 812-amino-acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDalpha and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.