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Effect of staurosporine on outflow facility in monkeys
B Tian1, B T Gabelt, P L Kaufman
1Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison 53792-3220, USA.
Purpose:
To determine the effect of the serine-threonine kinase inhibitor staurosporine on outflow facility in living monkeys.
Methods:
Total outflow facility was determined by two-level constant pressure perfusion of the anterior chamber bilaterally before and after intracameral infusion of staurosporine or vehicle in opposite eyes.
Results:
Intracameral staurosporine dose-dependently doubled outflow facility, with 0.1 microM, 1 microM, and 10 microM being subthreshold, effective, and maximal doses, respectively. At 50 microM, intracameral staurosporine was less effective than 10 microM on facility and induced corneal toxicity.
Conclusions:
The broad-spectrum protein kinase inhibitor staurosporine increases outflow facility in living monkeys, perhaps by affecting the trabecular meshwork cytoskeleton.