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Updated: Aug 8, 2026

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
Published on: September 11, 2016
A double-selective tissue culture system for isolation of wild-type poliovirus from sewage applied in a long-term
Y Manor1, R Handsher, T Halmut
1Central Virology Laboratory, Public Health Laboratories, Chaim Sheba Medical Center, Tel-Hashomer, Israel.
Abstract:
We describe a simple, cost-efficient, double-selective method for isolation of wild-type poliovirus from sewage samples containing vaccine polioviruses and other enteroviruses, with a detection limit of 18 to 50 PFU per 1 to 2 liters of sewage. By this method we were able to process 1,700 sewage samples collected between 1991 and 1996, from which 10,472 plaques were isolated, 41 of them being identified as wild-type polioviruses.
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