Cellulolytic enzymes in culture filtrates of Rhizoctonia lamellifera

During growth in a liquid culture containing a single soluble or an insoluble cellulosic carbon source, Rhizoctonia lamellifera released cellulolytic enzymes into the medium. These enzymes were separated by gel filtration and ion-exchange chromatography into seven components, three of high and four of low molecular weight. One of the components had the character of a C1 cellulase. When the components were combined they released more reducing sugars from cellulosic substrates than when used singly.