[Arylsulfatase activity in the pineal body, studied by electron microscopy]

For the demonstration of aryl-sulphatase in the corpus pineale as a substrat p-Nitrochatecholsulphat (2-Hydroxy-5-nitrophenyl sulphat) has been used. The reaction is negativ when p-Nitrophenylsulphat or acetylphenylsulphat are used. Localization of the enzyme occurred in the Golgi-system and in the smooth-surface endoplasmic reticulum. By the aid of the histochemical reaction the formation of primary lysosomes can be demonstrated on the level of the Golgi system and of the smooth-surfaced endoplasmic reticulum as well. Aryl-sulphatase correspond to the: A and B type. Reaction in the presence of pCMB and KCN is inhibited.