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Related Experiment Videos

Evaluation of an aseptic technique testing and challenge kit (ATTACK).

S Turco1, W H Miele, D Barnoski

  • 1Temple University School of Pharmacy, Philadelphia, PA.

Hospital Pharmacy
|January 1, 1993
PubMed
Summary

This study evaluated a kit for detecting microbial contamination in sterile pharmaceutical preparations. The kit effectively detected contamination, especially in suboptimal conditions, highlighting its utility in quality assurance.

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Area of Science:

  • Pharmaceutical quality assurance
  • Microbial contamination detection
  • Sterile pharmaceutical compounding

Background:

  • Growing concerns exist regarding quality assurance in sterile pharmaceutical compounding by healthcare professionals.
  • Accurate detection of microbial contamination is crucial for patient safety.

Purpose of the Study:

  • To assess the utility of a specific kit in detecting microbial contamination during simulated sterile drug transfers.
  • To evaluate the kit's performance under varying conditions and with aged media.

Main Methods:

  • Simulated drug transfers using syringes were performed.
  • Growth promotion studies utilized Trypticase Soy Broth with standard microbial test organisms (Staphylococcus epidermidis, Bacillus subtilis, Candida albicans) at varying concentrations.

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  • Kit performance was tested by trained and untrained individuals under aseptic and suboptimal conditions.

    • Main Results:

    • The kit's growth media supported 100% growth of inoculated organisms within 8 days, even with 32-month-old stored media.
    • Kits used with good aseptic technique showed no contamination.
    • Kits used in suboptimal environments by untrained individuals demonstrated a 30% contamination rate.

    Conclusions:

    • The evaluated kit is effective in detecting microbial contamination during simulated sterile pharmaceutical transfers.
    • The kit demonstrates reliability even with aged media and highlights the importance of aseptic technique and environmental control.