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Related Experiment Videos

Flash sterilization and instrument tape--an experimental study.

D A Kostyal1, J M Verhage, D H Beezhold

  • 1Donald Guthrie Foundation for Medical Research, Sayre, PA.

Journal of Healthcare Materiel Management
|September 5, 1993
PubMed
Summary
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Flash sterilization of instruments with color-coded tape is effective. Testing confirmed that 3-minute sterilization at 135°C kills spores beneath the tape, ensuring instrument sterility.

Area of Science:

  • Sterilization and Infection Control
  • Medical Device Reprocessing
  • Microbiology

Background:

  • Concerns were raised regarding the efficacy of flash sterilization for instruments with color identification tape.
  • A previous response suggested porous tape impedes sterilization and may lead to non-sterile areas if it detaches.
  • This assertion lacked experimental validation, necessitating empirical investigation.

Purpose of the Study:

  • To experimentally test the hypothesis that flash sterilization is effective for instruments with color identification tape.
  • To determine if spore kill is achieved on instrument surfaces beneath color-code tape during rapid sterilization cycles.

Main Methods:

  • Bacterial spores (B. stearothermophilus) were placed on discs positioned between color-code tape and a metal instrument.

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  • Instruments were subjected to flash sterilization using a gravity displacement sterilizer at 135°C for 3 minutes.
  • The presence or absence of microbial growth on the spore discs was assessed post-sterilization.

    • Main Results:

    • No bacterial growth was observed on any of the spore discs that were in contact with instruments under color-code tape.
    • This indicates complete spore kill was achieved in the areas shielded by the tape.

    Conclusions:

    • Sterility can be effectively achieved on instrument surfaces beneath color-code tape using a 3-minute flash sterilization cycle.
    • The study provides experimental evidence supporting the use of flash sterilization for color-coded instrument sets.