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Updated: Jul 17, 2026

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
Published on: August 3, 2021
The fluid double polar-nonpolar-polar leaflet model for biological membranes
Abstract:
A model for biological membranes is proposed according to which the plasma membrane consists of two functionally different polar-nonpolar-polar leaflets separated by a polar space. The binding of water-soluble proteins, integral lipoproteins and spanning proteins to a biological membrane as well as possible conformations of interphase peptides partitioned between polar and nonpolar layers are discussed. A model for the diffusion of water soluble proteins across nonpolar layers of a membrane is described. Three complete biological membranes containing two leaflets and an inter-leaflet space are defined. These are: 1: The inner nuclear membrane + the perinuclear space and the endoplasmatic cisternae + the outer nuclear membrane and the endoplasmatic reticulum, 2: the innner mitochondrial membrane + the mitochondrial intermembraneous space + the outer mitochondrial membrane and 3: the cytoplasmic leaflet of the plasma membrane + an intramembraneous space in the plasma membrane + the outer leaflet of the plasma membrane.
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