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Updated: Jul 25, 2026
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Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
Published on: November 4, 2016
Effective concentration of bone marrow mononuclear cells using density gradient separation within an automated cell
P K Baker1, E G Rhodes, J K Duguid
1Royal Liverpool University Hospital Trust, U.K.
Transfusion Science
|June 7, 1992
Summary
This study presents an effective bone marrow concentration method using a cell separator and density medium, enhancing mononuclear cell recovery and successful engraftment after processing.
Area of Science:
- Hematology
- Cell Separation Technology
Background:
- Bone marrow concentration is crucial for hematopoietic stem cell transplantation.
- Current methods may involve extensive manipulation, risking cell viability and contamination.
Purpose of the Study:
- To describe an effective method for concentrating bone marrow using a cell separator.
- To enhance mononuclear cell recovery and reduce mature hematopoietic cell contamination.
Main Methods:
- Utilized an intermittent flow cell separator for bone marrow concentration.
- Incorporated a density separation medium (Ficoll-metrizoate) to eliminate mature cells.
- Washing steps were performed within the cell separator to minimize manipulation.
Main Results:
- Achieved 86.8% depletion of mature granulocytes and 97.7% depletion of red blood cells.
- Reported a 49% recovery rate for mononuclear cells.
- Processed bone marrow demonstrated successful engraftment post-purging/cryopreservation.
Conclusions:
- The described method provides standardized, efficient bone marrow concentration.
- This technique enhances mononuclear cell recovery and reduces contamination risks.
- The processed bone marrow is suitable for subsequent therapeutic applications like transplantation.
