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Related Experiment Videos

Accuracy and precision of a robotic sample processor.

D A Armbruster1, L C Hawes, C T Winter

  • 1Armstrong Laboratory, Human Systems Center, Brooks Air Force Base, Texas 78235.

Journal of the International Federation of Clinical Chemistry
|August 6, 1992
PubMed
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The Packard Probe 1000 automated liquid handler demonstrates high accuracy and precision for sample processing. Calibration is crucial, as performance varies with liquid type, volume, and pipetting mode.

Area of Science:

  • Biotechnology
  • Laboratory Automation
  • Analytical Chemistry

Background:

  • Automated liquid handling systems are essential for high-throughput laboratory operations.
  • Accurate and precise sample processing is critical for reliable experimental results.
  • The Packard Probe 1000 is a robotic sample processor requiring performance validation.

Purpose of the Study:

  • To evaluate the accuracy and precision of the Packard Probe 1000 robotic sample processor.
  • To determine performance characteristics across different liquid types, volumes, and pipetting modes.
  • To assess the suitability of the system for clinical and research applications.

Main Methods:

  • Gravimetric procedure following NCCLS Guideline 18-P.
  • Testing of multiple liquid volumes (25, 100, 200 muL) with saline and human serum.

Related Experiment Videos

  • Evaluation of 'dip and sip' and 'dispense-through' pipetting modes.
  • Adjustment of instrument operating parameters from factory defaults.

    • Main Results:

    • Inaccuracy ranged from -0.5% to +0.7% across tested conditions.
    • Imprecision ranged from 0.02% to 0.8% across tested conditions.
    • Optimal performance required adjustment of factory default settings.
    • Inaccuracy and imprecision below 1.0% were achievable.

    Conclusions:

    • The Packard Probe 1000 offers impressive accuracy and precision for automated liquid handling.
    • Performance is dependent on liquid characteristics, volume, and pipetting mode.
    • Calibration is essential, similar to any volumetric device, for reliable operation.