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A nondenaturing solid phase pharmaceutical carrier comprised of surface-modified nanocrystalline materials
N Kossovsky1, R F Bunshah, A Gelman
1Department of Pathology and Laboratory Medicine, University of California, Los Angeles 90024.
Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials
|January 1, 1990
Summary
Researchers developed a novel, non-denaturing carrier using nanocrystalline tin oxide and an organic bonding layer. This carrier successfully maintained the conformation of bound proteins, showing promise for drug delivery systems and vaccines.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Drug Delivery Systems
Background:
- Controlled delivery systems often risk denaturing active molecules.
- Biophysical interactions between carriers and molecules can reduce efficacy.
Purpose of the Study:
- To synthesize a non-denaturing carrier system.
- To evaluate the carrier's ability to preserve protein conformation.
Main Methods:
- Synthesized a nanocrystalline tin oxide core with a GF292 organic bonding layer.
- Bound human transferrin to the carrier.
- Assessed protein conformation using immunoelectron microscopy.
Main Results:
- The synthesized carrier maintained the structural integrity of human transferrin.
- Immunoelectron microscopy confirmed retained protein conformation.
Conclusions:
- Nanocrystalline cores with surface-modifying agents are suitable for creating non-denaturing carriers.
- This approach holds potential for developing targeted drug systems and vaccines.