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Related Experiment Videos

Continuous flow cell separator use for bone marrow processing.

P K Baker1, E G Rhodes, J K Duguid

  • 1Department of Haematology, Royal Hospital, Liverpool, England.

Transfusion Science
|December 10, 1990
PubMed
Summary
This summary is machine-generated.

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This study demonstrates a new method for processing bone marrow for transplantation using the COBE Spectra cell separator. This technique efficiently isolates mononuclear cells and hematopoietic stem cells, ensuring successful patient engraftment.

Area of Science:

  • Hematology
  • Cellular Biology
  • Transplantation Medicine

Background:

  • Bone marrow processing is crucial for transplantation, purging, and cryopreservation.
  • Effective methods often involve centrifugation and density separation for optimal marrow concentrate.
  • Continuous flow cell separation offers potential advancements in marrow processing.

Purpose of the Study:

  • To evaluate the efficacy of the COBE Spectra continuous flow cell separator for bone marrow processing.
  • To assess the ability of this technology to rapidly collect an ideal marrow concentrate.
  • To determine the impact of an inert sedimenting agent on cell recovery and purity.

Main Methods:

  • Utilized the COBE Spectra continuous flow cell separator for bone marrow processing.

Related Experiment Videos

  • Incorporated an inert sedimenting agent prior to processing to enhance differential cell collection.
  • Employed centrifugation and density separation principles within the cell separator's algorithm control.

    • Main Results:

    • Achieved a significant volume depletion of 87% during marrow processing.
    • Recovered 76.4% of mononuclear cells and 86.5% of CFU-GM progenitor cells.
    • Processed marrow resulted in successful transplantation with no delay in engraftment and sustained grafts.

    Conclusions:

    • The COBE Spectra system, with its advanced technology and algorithms, provides rapid and effective bone marrow processing.
    • The addition of a sedimenting agent improves the isolation of mononuclear and progenitor cells while removing unwanted cell types.
    • This processing technique yields a high-quality marrow concentrate suitable for successful hematopoietic stem cell transplantation.