Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ribonucleic acid synthesis in rabbit erythroid cells.

J A Hunt

    The Biochemical Journal
    |December 15, 1976
    PubMed
    Summary

    This study quantifies RNA synthesis rates in rabbit bone-marrow erythroid cells, revealing distinct synthesis and processing kinetics for poly(A)-containing RNA, ribosomal RNA, and nuclear RNA.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    SEA URCHIN DNA SEQUENCE VARIATION AND REDUCED INTERSPECIES DIFFERENCES OF THE LESS VARIABLE DNA SEQUENCES.

    Evolution; international journal of organic evolution·2017
    Same author

    A scientific evidence for the efficacy of biologic implants for soft tissue reconstruction.

    Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2012
    Same author

    Anaerobic co-digestion of sewage sludge and primary clarifier skimmings for increased biogas production.

    Water science and technology : a journal of the International Association on Water Pollution Research·2012
    Same author

    In vitro activation of human leukocytes in response to contact with synthetic hernia meshes.

    Clinical biochemistry·2012
    Same author

    The role of calcified cartilage and subchondral bone in the initiation and progression of ochronotic arthropathy in alkaptonuria.

    Arthritis and rheumatism·2011
    Same author

    The use of dynamic surface chemistries to control msc isolation and function.

    Biomaterials·2011

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Biochemistry

    Background:

    • Understanding RNA synthesis is crucial for cellular function.
    • Erythroid cells provide a model for studying specific gene expression.
    • Previous studies lacked detailed kinetic data on RNA synthesis in these cells.

    Purpose of the Study:

    • To kinetically analyze RNA synthesis in mature rabbit bone-marrow erythroid cells.
    • To determine the synthesis rates and half-lives of different RNA types.
    • To compare these rates with those in other cell types.

    Main Methods:

    • Incorporation of [2-3H]adenosine into ATP pool and RNA.
    • Mathematical modeling of pool specific radioactivity and DNA synthesis.
    • Curve fitting to determine RNA synthesis rates and processing half-lives.

    Main Results:

    • Poly(A)-containing RNA synthesis rate: 90-220 fmol/min/cell; nuclear processing half-life: 16 min.
    • Ribosomal RNA synthesis rate: 70-200 fmol/min/cell; processing half-lives: 37 min (18S) and 214 min (28S).
    • Nuclear RNA synthesis rate: 2-6 fg/min/cell; degradation half-life: 27 min.

    Conclusions:

    • Distinct kinetic parameters were established for various RNA classes in erythroid cells.
    • Poly(A)-containing RNA synthesis represents a small fraction of total RNA synthesis.
    • Findings provide a quantitative basis for comparing RNA metabolism across different cell types.

    Related Experiment Videos