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An improved method for PHA-culturing mouse lymphocytes using Ficoll-Hypaque gradient
A simple, reproducible method is described for in vitro culture of mouse lymphocytes purified by differential centrifugation through Ficoll-Hypaque gradient. Peripheral blood, spleen and thymus purified lymphocytes responded well to in vitro PHA stimulation as measured by blast transformation and tritiated thymidine incorporation; the lowest values were observed with thymus lymphocytes.
A simple, reproducible method is described for in vitro culture of mouse lymphocytes purified by differential centrifugation through Ficoll-Hypaque gradient. Peripheral blood, spleen and thymus purified lymphocytes responded well to in vitro PHA stimulation as measured by blast transformation and tritiated thymidine incorporation; the lowest values were observed with thymus lymphocytes.