Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

IVBT-documented platelet function correlates with flow cytometric data.

J Hoffmann1, G Bonacker, V Kretschmer

  • 1Department of Transfusion Medicine and Haemostaseology, University Hospital, Marburg, Germany.

Transfusion Science
|November 3, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic accuracy of RISK6 assay in childhood pulmonary TB.

IJTLD open·2025
Same author

Non-sputum-based triage and confirmatory diagnostic tests for pediatric TB.

IJTLD open·2025
Same author

Addressing gaps in the diagnosis of TB in children.

IJTLD open·2024
Same author

Stool Xpert<sup>®</sup> MTB/RIF Ultra for TB diagnosis in children: experience from a national scale-up programme.

IJTLD open·2024
Same author

[Acceptance of psychosocial bridging measures in dementia : Treatment observation during the COVID-19 pandemic].

Zeitschrift fur Gerontologie und Geriatrie·2022
Same author

Photogrammetric evaluation of corrective surgery for trigonocephaly.

International journal of oral and maxillofacial surgery·2021

Platelet function, not just count, impacts bleeding risk in thrombocytopenic patients. Flow cytometry revealed that impaired platelet function correlates with reduced expression of collagen and thrombin receptors.

Area of Science:

  • Hematology
  • Immunology
  • Biochemistry

Background:

  • Thrombocytopenic patients exhibit variable bleeding tendencies despite similar platelet counts.
  • Platelet function significantly influences bleeding risk, independent of platelet count.
  • In vitro bleeding tests (IVBT) can quantify bleeding tendencies.

Purpose of the Study:

  • To investigate platelet antigen expression using flow cytometry to explain functional differences.
  • To correlate platelet antigen expression with in vitro bleeding test results and clinical bleeding scores.
  • To assess the impact of platelet transfusion on antigen expression and transfusion efficacy.

Main Methods:

  • Flow cytometry was used to evaluate platelet-rich plasma from 30 thrombocytopenic patients.

Related Experiment Videos

  • Monoclonal antibodies against GP IIIb (collagen receptor), GP IIb/IIIa (fibrinogen receptor), and GP Ib (thrombin receptor) were utilized.
  • Platelet populations were categorized based on in vitro bleeding test results (occlusion time and Simplate time) and clinical bleeding scores.

    • Main Results:

    • Impaired platelet function (longer occlusion/Simplate times) correlated with significantly lower expression of GP IIIb and GP Ib.
    • Patients with severe neutropenia (<1000 WBC/microliter) showed reduced GP IIIb and GP Ib expression.
    • Platelet transfusion increased the expression of GP IIIb and GP Ib, correlating with better transfusion efficacy.
    • Higher bleeding scores were associated with lower GP Ib expression.

    Conclusions:

    • In vitro bleeding test-assessed platelet function is directly related to the expression levels of collagen and thrombin receptors on platelets.
    • Platelet antigen expression, particularly GP IIIb and GP Ib, serves as a potential biomarker for platelet function and transfusion efficacy.
    • Flow cytometry offers a valuable tool for characterizing platelet function in thrombocytopenic patients.